Detection dogs trained to sniff out the scat of an endangered lizard in California's San Joaquin Valley, combined with genetic species identification, could represent a new noninvasive sampling technique for lizard conservation worldwide. That is according to a study published today from the University of California, Davis, in partnership with the nonprofit Working Dogs for Conservation, U.S. Geological Survey and the U.S. Bureau of Land Management.
Scientists have used trained conservation dogs to locate scat and collect DNA samples for everything from bears and foxes to gorillas and whales. But the technique had not been used for reptiles until this study, for which scientists developed a novel approach to identify the presence of the blunt-nosed leopard lizard in the Panoche Hills Recreation Area and Carrizo Plain National Monument, both managed by BLM.
They developed new methods to recover DNA from feces and genetically identify lizard species in the same area. The study, published in the Journal of Wildlife Management, is a proof of concept for a host of reptiles.
"So many reptilian species have been hit so hard," said lead author Mark Statham, an associate researcher with the Mammalian Ecology and Conservation Unit of the UC Davis School of Veterinary Medicine. "A large proportion of them are endangered or threatened. This is a really valuable way for people to be able to survey them."
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Current methods for surveying lizard species typically rely on live capture or visual surveys. Scat sampling allows biologists to study elusive, rare or dangerous animals without the need for direct contact. In addition to informing about the presence, habitat and genetics of an animal, scat can also be analyzed to inform researchers about diet, hormones, parasites and other health factors.
Using the new method, the authors genetically identified specific species for 78 percent of the 327 samples collected by dog-handler teams across four years. Most (82 percent) of those identified were confirmed as being from blunt-nosed leopard lizards.
To meet regulatory monitoring requirements, more research is needed to assess the viability of using detection dogs to recover usable DNA at larger scales. But the research highlights the broad potential this method holds for surveying and monitoring reptiles.
Study coauthors include Deborah A. Woollett, Alice Whitelaw and Ngaio L. Richards of Working Dogs for Conservation; Susan Fresquez, Jerene Pfeiffer and Benjamin Sacks from UC Davis School of Veterinary Medicine; Jonathan Richmond from the U.S. Geological Survey; and Michael F. Westphal of the U.S. Bureau of Land Management.
Funding was provided by the Bureau of Land Management.
Story Source:
Materials provided by University of California - Davis. Original written by Kat Kerlin. Note: Content may be edited for style and length.
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